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anti wnt2  (Proteintech)


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    Proteintech anti wnt2
    Anti Wnt2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+wnt2/pmc13028182-24-0-2?v=Proteintech
    Average 94 stars, based on 27 article reviews
    anti wnt2 - by Bioz Stars, 2026-08
    94/100 stars

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    The relative expression of <t>Wnt2b,</t> one of the key proteins in the Wnt signaling pathway, was detected by RT-qPCR in RAW264.7 cells, during treatment with (A) LIPUS and LPS or (B) LIPUS and IL4. RAW indicates RAW264.7 cells used as control. RAW-LPS indicates RAW264.7 cells treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS indicates RAW264.7 cells treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LIPUS indicates RAW264.7 cells treated with low-intensity pulsed ultrasound. RAW+IL4 indicates RAW264.7 cells treated with 20 ng/mL IL4 to induce macrophage-like M2 polarization. RAW+IL4+LIPUS indicates RAW264.7 cells treated with 20 ng/mL IL4 and low-intensity pulsed ultrasound. * P < 0.05, ** P < 0.005, **** P < 0.0001.
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    The relative expression of Wnt2b, one of the key proteins in the Wnt signaling pathway, was detected by RT-qPCR in RAW264.7 cells, during treatment with (A) LIPUS and LPS or (B) LIPUS and IL4. RAW indicates RAW264.7 cells used as control. RAW-LPS indicates RAW264.7 cells treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS indicates RAW264.7 cells treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LIPUS indicates RAW264.7 cells treated with low-intensity pulsed ultrasound. RAW+IL4 indicates RAW264.7 cells treated with 20 ng/mL IL4 to induce macrophage-like M2 polarization. RAW+IL4+LIPUS indicates RAW264.7 cells treated with 20 ng/mL IL4 and low-intensity pulsed ultrasound. * P < 0.05, ** P < 0.005, **** P < 0.0001.

    Journal: PeerJ

    Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

    doi: 10.7717/peerj.18448

    Figure Lengend Snippet: The relative expression of Wnt2b, one of the key proteins in the Wnt signaling pathway, was detected by RT-qPCR in RAW264.7 cells, during treatment with (A) LIPUS and LPS or (B) LIPUS and IL4. RAW indicates RAW264.7 cells used as control. RAW-LPS indicates RAW264.7 cells treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS indicates RAW264.7 cells treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LIPUS indicates RAW264.7 cells treated with low-intensity pulsed ultrasound. RAW+IL4 indicates RAW264.7 cells treated with 20 ng/mL IL4 to induce macrophage-like M2 polarization. RAW+IL4+LIPUS indicates RAW264.7 cells treated with 20 ng/mL IL4 and low-intensity pulsed ultrasound. * P < 0.05, ** P < 0.005, **** P < 0.0001.

    Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

    Techniques: Expressing, Quantitative RT-PCR, Control

    (A) CD80 and (B) CD86 were detected by flow cytometry during M1 polarization of RAW264.7 cells. RAW+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with low-intensity pulsed ultrasound. *** P < 0.0005, **** P < 0.0001.

    Journal: PeerJ

    Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

    doi: 10.7717/peerj.18448

    Figure Lengend Snippet: (A) CD80 and (B) CD86 were detected by flow cytometry during M1 polarization of RAW264.7 cells. RAW+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with low-intensity pulsed ultrasound. *** P < 0.0005, **** P < 0.0001.

    Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

    Techniques: Flow Cytometry, Transfection, Negative Control, Control

    Relative expression of (A) Wnt2b, (B) AXIN, and (C) β-catenin detected by Western blot. RAW-Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. **** P < 0.0001.

    Journal: PeerJ

    Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

    doi: 10.7717/peerj.18448

    Figure Lengend Snippet: Relative expression of (A) Wnt2b, (B) AXIN, and (C) β-catenin detected by Western blot. RAW-Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. **** P < 0.0001.

    Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

    Techniques: Expressing, Western Blot, Transfection, Negative Control, Control

    (A) The expression of IL2, IL4, IL6, IL10, TNFα, IFNγ, and IL17A were detected by flow cytometry. (B) The expression of IL6 and TNFα was increased by the downregulation of Wnt2b during LIPUS and LPS treatment. RAW+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. * P < 0.05, **** P < 0.0001.

    Journal: PeerJ

    Article Title: Anti-inflammatory role of low-intensity pulsed ultrasound in inhibiting lipopolysaccharide-induced M1 polarization of RAW264.7 cells via Wnt2b/AXIN/β-catenin

    doi: 10.7717/peerj.18448

    Figure Lengend Snippet: (A) The expression of IL2, IL4, IL6, IL10, TNFα, IFNγ, and IL17A were detected by flow cytometry. (B) The expression of IL6 and TNFα was increased by the downregulation of Wnt2b during LIPUS and LPS treatment. RAW+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA, used as control. RAW+LPS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS to induce macrophage-like M1 polarization. RAW+LPS+LIPUS+Wnt2bsiNC indicates RAW264.7 cells transfected with negative control of Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. RAW+LPS+LIPUS+Wnt2bsi1/2/3 indicates RAW264.7 cells transfected with Wnt2b siRNA and treated with 100 ng/mL LPS and low-intensity pulsed ultrasound. * P < 0.05, **** P < 0.0001.

    Article Snippet: Primary antibodies used in the study included Wnt2b (66656-1-Ig; Proteintech, CA, Rosemont, IL, USA), AXIN (20540-1-AP; Proteintech, Rosemont, Illinois, China), β-catenin (51067-2-AP; Proteintech, Rosemont, IL, USA), and GAPDH (10494-1-AP; Proteintech, Rosemont, IL, USA).

    Techniques: Expressing, Flow Cytometry, Transfection, Negative Control, Control